Fig. 1

Reaction scheme of cellulose phosphorolysis. a Cellulosic biomass was enzymatic phosphorylated via in vitro synthetic enzymatic biosystems to manufacturing biochemicals or biofuels. b In the free enzyme mixture, CDP prefers to convert cellodextrin with a degree of polymerization of n − 1 (G_(n−1)) and G1P to G_(n) and inorganic phosphate (P_i) despite the presence of PGM. c The enzyme complex containing CDP and PGM was supposed to accelerate the degradation of cellodextrin to G6P. CDP cellodextrin phosphorylase, PGM phosphoglucomutase, G_(n) and G_(n−1) cellodextrin, G1P glucose 1-phosphate, G6P glucose 6-phosphate, P_i inorganic phosphate