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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: Acceleration of cellodextrin phosphorolysis for bioelectricity generation from cellulosic biomass by integrating a synthetic two-enzyme complex into an in vitro synthetic enzymatic biosystem

Fig. 2

Schematic representation of the chimeric enzymes and four types of enzyme complexes. a Schematic representation of dockerin-tagged recombinant proteins and cohesin-containing scaffoldin. Two dockerins containing CDPs (part 1 and 2), two dockerins containing PGM (part 3 and 4), and a synthetic scaffoldin (part 5) were constructed. b Types of synthetic enzyme complexes. The type I CDP–PGM complex was constructed by CDP-CtDoc, PGM-CcsDoc and CBM3-Scaf3 (parts 1 + 3 + 5); the type II complex was constructed by CDP-RfDoc, PGM-CcsDoc and CBM3-Scaf3 (parts 2 + 3 + 5); the type III complex was constructed by CDP-CtDoc, PGM-RfDoc and CBM3-Scaf3 (parts 1 + 4 + 5); and the type IV complex was constructed by CDP-CtDoc, CDP-RfDoc, PGM-CcsDoc and CBM3-Scaf3 (parts 1 + 2 + 3 + 5)

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