Fig. 5

Electrochemical performance of the cellodextrin-powered in vitro synthetic enzymatic biosystem catalyzed by synthetic enzyme complexes and enzyme mixture. a Reaction scheme of the in vitro synthetic pathway of the cellodextrin-powered biosystem for bioelectricity generation. CDP cellodextrin phosphorylase, PGM phosphoglucomutase, G6PDH glucose 6-phosphate dehydrogenase, 6PGDH 6-phosphogluconate dehydrogenase, DI diaphorase, G6P glucose 6-phosphate, 6PG 6-phosphogluconate, Ru5P ribulose 5-phosphate, AQDS_Red reduced mediator, AQDS_OX oxidized mediator. b Amperometric response of the cellodextrin-powered biosystems containing synthetic enzyme complex and enzyme mixture in the initial stage of the reaction. c Polarization curves of the cellodextrin-powered biosystems containing synthetic enzyme complex and enzyme mixture. d Profiles of power density versus current of the cellodextrin-powered biosystems containing synthetic enzyme complex and enzyme mixture. Type I enzyme complex (red line) was loaded under the enzyme unit of 8.64 U L⁻¹ CDP and 284 U L⁻¹ PGM. Type IV enzyme complex (green line) was loaded under the enzyme unit of 17.3 U L⁻¹ CDP and 284 U L⁻¹ PGM. Free enzyme mixture was loaded under the enzyme unit of 8.64 U L⁻¹ CDP and 284 U L⁻¹ PGM (black line), 17.3 U L⁻¹ CDP and 284 U L⁻¹ PGM (dark yellow line), 34.6 U L⁻¹ CDP and 284 U L⁻¹ PGM (dark cyan line), and 104 U L⁻¹ CDP and 284 U L⁻¹ PGM (magenta line), respectively. Values shown are means of triplicate determinations