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Table 1 Apparent kinetic parameters for the type I synthetic enzyme complex and enzyme mixture at 60 °C

From: Acceleration of cellodextrin phosphorolysis for bioelectricity generation from cellulosic biomass by integrating a synthetic two-enzyme complex into an in vitro synthetic enzymatic biosystem

Name

Cellodextrin

Cellopentose

Km (g L−1)

kcat (s−1)

kcat/Km (s−1 g−1 L)

Km (mM)

kcat (s−1)

kcat/Km (mM−1 s−1)

Type I CDP–PGM complex

2.1 ± 0.7

3.9 ± 0.4

1.9

1.9 ± 0.2

4.6 ± 0.2

2.5

Enzyme mixture

2.8 ± 0.3

1.5 ± 0.1

0.6

2.3 ± 0.2

1.7 ± 0.1

0.7