Table 2 Primers used in this study
Primer | Sequence (5′–3′) | Template |
|---|---|---|
VF-CDP-CtDoc | acatatagtgactcttaagtttaaagttgattggaacaaggcaactgctt | pET20b-tim-ctdoc |
VR-CDP-CtDoc | aagagcgacaatgaaatcattagtatacatatagaggaagaatttcaatt | |
IF-CDP-CtDoc | ttaactttaagaaggagatatacatatgattactaaagtaacagcgagaa | pET21c-ctcdp |
IR-CDP-CtDoc | ttcgtcaacggaacaaggttagttgaaatttgaattctcagtgatataca | |
VF-CDP-RfDoc | acatatagtgactcttaagtttaaacccggcacaaagctcgttcctacat | pET20b-fbp-rfdoc |
VR-CDP-RfDoc | The same with VR-CDP-CtDoc | |
IF-CDP-RfDoc | The same with IF-CDP-CtDoc | pET21c-ctcdp |
IR-CDP-RfDoc | tacatccttgctcgaaacacggcccaaatttgaattctcagtgatataca | |
VF-PGM-CcsDoc | actgctggaagaagcactgaaaggtggtaaggtattaccaggaatccaag | pET20b-ald-ccsdoc |
VR-PGM-CcsDoc | gcttccatggtttgtcaaacgggtatacatatagaggaagaatttcaatt | |
IF-PGM-CcsDoc | ttaactttaagaaggagatatacatatgggcaaactgtttggtaccttcg | pET20b-tkpgm |
IR-PGM-CcsDoc | gaacctaaggaccattatggaatggtggaaagtcacgaagaaggtcgtca | |
VF-PGM-RfDoc | actgctggaagaagcactgaaaggtcccggcacaaagctcgttcctacat | pET20b-fbp-rfdoc |
VR-PGM-RfDoc | The same with VR-PGM-CcsDoc | |
IF-PGM-RfDoc | The same with IF-PGM-CcsDoc | pET20b-tkpgm |
IR-PGM-RfDoc | tacatccttgctcgaaacacggccctggaaagtcacgaagaaggtcgtca |