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Table 3 Plasmids used in this study

From: Acceleration of cellodextrin phosphorolysis for bioelectricity generation from cellulosic biomass by integrating a synthetic two-enzyme complex into an in vitro synthetic enzymatic biosystem

PlasmidsCharacteristicsRefs.
pET20b-cbm3-scaf3AmpR, mini-scaffoldin expression cassette containing a CBM3 module from C. thermocellum CipA and three different cohesins from C. thermocellum, C. cellulovorans and R. flavefaciens[40]
pET21c-ctcdpAmpR, ctcdp expression cassette containing CDP, which was purified based on the C-terminal 6× His tag[37]
pET20b-tkpgmAmpR, tkpgm expression cassette containing PGM, which was purified based on the C-terminal 6× His tag[10]
pET20b-ctcdp-ctdocAmpR, ctcdp-ctdoc expression cassette containing CDP from T. thermophiles and the dockerin module from C. thermocellumThis work
pET20b-ctcdp-rfdocAmpR, ctcdp-rfdoc expression cassette containing CDP module from T. maritime and the dockerin module from R. flavefaciensThis work
pET20b-tkpgm-ccsdocAmpR, tkpgm-ccsdoc expression cassette containing PGM module from T. maritime and the dockerin module from C. cellulovoransThis work
pET20b-tkpgm-rfdocAmpR, tkpgm-rfdoc expression cassette containing PGM module from T. maritime and the dockerin module from R. flavefaciensThis work