Fig. 1
From: Re-routing photosynthetic energy for continuous hydrogen production in vivo
Construction and screening of HS mutants. a Plasmid vector map of pchlamy1 Hyd–SOD. b R. capsulatus screen (Rhodo assay) for HydA–SOD-expressing clones. Algae chlorophyll fluorescence is green whereas dark halation represents GFP produced by R. capsulatus due to H2 presence. The WT strain CC-124 (High), Fd–HydA-expressing clones (Pos.) and DM (Neg.) clones were used as positive, transformant reference and negative controls, respectively. Numbers represent HS clones. c PCR analysis of genomic DNA from DM, HS-14, HS-40 and HS-62 cells. Primers for pChlmy1 rbcS promotor (forward) and the 3′UTR (reverse) were used and the expected amplified chain length is 2400 bp. d Immunoblot analysis for the detection of Hyd–SOD expression. Soluble fraction [S] and whole cell protein extraction [T] from DM and HS-14 were loaded on 4–12% Bis–Tris PAGE (Life Technologies) and probed with rabbit polyclonal HydA1/2 antibodies and ponceau red