Skip to main content

Table 1 Overview of kinetic parameters of BxlB glycomutants

From: Redesigning N-glycosylation sites in a GH3 β-xylosidase improves the enzymatic efficiency

 

Km (mM)

Vmax (U/mg)

kcat (1/s)

kcat/Km

kcat/Km (%)a

BxlBwt

2.06 ± 0.13

9.55 ± 0.18

13.08

6.34

100

BxlBN1;5;7

2.49 ± 0.43

17.28 ± 1.07

23.67

9.51

150

BxlBnon-glyc

2.36 ± 0.11

10.12 ± 0.14

13.86

5.87

93

BxlBN1

2.53 ± 0.23

9.49 ± 0.28

13.01

5.13

81

BxlBN5

2.71 ± 0.21

6.08 ± 016

3.71

0.61

10

BxlBN7

3.19 ± 0.22

3.42 ± 0.09

4.37

1.27

20

BxlBN5;7

2.81 ± 0.11

20.47 ± 0.28

28.04

9.94

157

BxlBN1;5

2.91 ± 0.18

21.95 ± 0.51

30.07

10.33

163

BxlBN1;7

9.92 ± 1.05

4.12 ± 0.26

5.64

0.57

9

  1. aRelative to BxlBwt