Skip to main content

Table 1 Overview of kinetic parameters of BxlB glycomutants

From: Redesigning N-glycosylation sites in a GH3 β-xylosidase improves the enzymatic efficiency

  Km (mM) Vmax (U/mg) kcat (1/s) kcat/Km kcat/Km (%)a
BxlBwt 2.06 ± 0.13 9.55 ± 0.18 13.08 6.34 100
BxlBN1;5;7 2.49 ± 0.43 17.28 ± 1.07 23.67 9.51 150
BxlBnon-glyc 2.36 ± 0.11 10.12 ± 0.14 13.86 5.87 93
BxlBN1 2.53 ± 0.23 9.49 ± 0.28 13.01 5.13 81
BxlBN5 2.71 ± 0.21 6.08 ± 016 3.71 0.61 10
BxlBN7 3.19 ± 0.22 3.42 ± 0.09 4.37 1.27 20
BxlBN5;7 2.81 ± 0.11 20.47 ± 0.28 28.04 9.94 157
BxlBN1;5 2.91 ± 0.18 21.95 ± 0.51 30.07 10.33 163
BxlBN1;7 9.92 ± 1.05 4.12 ± 0.26 5.64 0.57 9
  1. aRelative to BxlBwt