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Table 1 Overview of kinetic parameters of BxlB glycomutants

From: Redesigning N-glycosylation sites in a GH3 β-xylosidase improves the enzymatic efficiency

 Km (mM)Vmax (U/mg)kcat (1/s)kcat/Kmkcat/Km (%)a
BxlBwt2.06 ± 0.139.55 ± 0.1813.086.34100
BxlBN1;5;72.49 ± 0.4317.28 ± 1.0723.679.51150
BxlBnon-glyc2.36 ± 0.1110.12 ± 0.1413.865.8793
BxlBN12.53 ± 0.239.49 ± 0.2813.015.1381
BxlBN52.71 ± 0.216.08 ± 0163.710.6110
BxlBN73.19 ± 0.223.42 ± 0.094.371.2720
BxlBN5;72.81 ± 0.1120.47 ± 0.2828.049.94157
BxlBN1;52.91 ± 0.1821.95 ± 0.5130.0710.33163
BxlBN1;79.92 ± 1.054.12 ± 0.265.640.579
  1. aRelative to BxlBwt