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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: The use of lytic polysaccharide monooxygenases in anaerobic digestion of lignocellulosic materials

Fig. 3

Cumulative methane production during anaerobic digestion of birch with addition of Cellic Ctec2 (a), Celluclast (b), NcLPMO9C (c) and an 85% Celluclast and 15% NcLPMO9C (Cell + 9C) blend (d). The microbial inoculum was collected from a cow-manure-and-food-waste fed biogas reactor. Enzymes were supplied once at day 0 at 4 mg of protein per gram of substrate, with and without following addition of H2O2. Where indicated, hydrogen peroxide was supplied at 0 h, 24 h, 48 h and 72 h at 0.1 mM final concentration. Deionized water was added in all reactions without H2O2. Boiled enzyme control reactions with added H2O2 are also shown. Inoculum methane release was subtracted from all samples in the calculation of methane production. The curves represent the average of two separate experiments. Methane production rates for these experiments are shown in Additional file 1: Figure S3

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