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Table 1 Initial Glc4gemGlc concentrations in biogas reactions with Avicel supplemented with commercial cellulase cocktails (Cellic Ctec2 or Celluclast) or purified NcLPMO9C as well as H2O2

From: The use of lytic polysaccharide monooxygenases in anaerobic digestion of lignocellulosic materials

SampleGlc4gemGlc (µM)
Anaerobic reactionsaAerobic reactions
1 min1 h4 h24 h1 min1 h4 h24 h
Control-Inoculum + H2O2ndndndndndndndnd
Avicel + H2O2ndndndndndndndnd
Avicel + Ctec2 + H2O2nd0.6ndndnd1.12.8nd
Avicel + Celluclast + H2O2ndndndndndnd0.8nd
Avicel + NcLPMO9C + H2O25.3b1.41.4nd8.42.82.5nd
Avicel + Ctec2ndndndndnd1.42.5nd
Avicel + Celluclastndndndndndnd0.8nd
Avicel + NcLPMO9C9.5b1.70.6nd9.83.43.1nd
Avicel + boiledCtec2 + H2O2ndndndndndndndnd
Avicel + boiledCelluclast + H2O2ndndndndndndndnd
Avicel + boiledNcLPMO9C + H2O2ndndndndndndndnd
  1. The headspace of the reactions contained nitrogen (anaerobic) or air (aerobic). The microbial inoculum was collected from a food-waste-and-cow-manure fed laboratory reactor. Enzymes were supplied once at time 0 at 4 mg of protein/g of substrate. Hydrogen peroxide was supplied at 0 h and 24 h (1 min before sampling at vigorous stirring) at 0.1 mM final concentration. The same volume of deionized water was added in all reactions without H2O2. Boiled enzyme control reactions plus H2O2 were also included. nd not detected
  2. aOnly the headspace was sparged with N2, meaning that some oxygen was present in the reaction mixture
  3. bChromatograms are shown in Additional file 1: Figure S1