The use of lytic polysaccharide monooxygenases in anaerobic digestion of lignocellulosic materials

Table 1 Initial Glc4gemGlc concentrations in biogas reactions with Avicel supplemented with commercial cellulase cocktails (Cellic Ctec2 or Celluclast) or purified NcLPMO9C as well as H₂O₂

The headspace of the reactions contained nitrogen (anaerobic) or air (aerobic). The microbial inoculum was collected from a food-waste-and-cow-manure fed laboratory reactor. Enzymes were supplied once at time 0 at 4 mg of protein/g of substrate. Hydrogen peroxide was supplied at 0 h and 24 h (1 min before sampling at vigorous stirring) at 0.1 mM final concentration. The same volume of deionized water was added in all reactions without H₂O₂. Boiled enzyme control reactions plus H₂O₂ were also included. nd not detected
^aOnly the headspace was sparged with N₂, meaning that some oxygen was present in the reaction mixture
^bChromatograms are shown in Additional file 1: Figure S1

ISSN: 2731-3654