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Table 3 Initial Glc4gemGlc concentrations in biogas reactions with birch, spruce and lignin-rich residue from birch (LRR) supplemented with purified NcLPMO9C or 85% Celluclast plus 15% NcLPMO9C (Cell + 9C)

From: The use of lytic polysaccharide monooxygenases in anaerobic digestion of lignocellulosic materials

Sample:aGlc4gemGlc (µM)
1 min1 h4 h24 h
Birch-NcLPMO9C + H2O23.90.32.5b0.6
Birch + NcLPMO9C3.40.3nd0.6
Spruce + NcLPMO9C + H2O2ndndnd0.8
Spruce + NcLPMO9Cndnd2.5bnd
LRR + NcLPMO9C + H2O2ndndnd0.3
LRR + NcLPMO9Cndndnd0.6
Birch- Cell + 9C +H2O2ndnd2.8b0.8
Birch + Cell + 9Cndndnd1.1
Spruce + Cell + 9C +H2O2ndndnd1.1
Spruce + Cell + 9Cndnd2.0b0.8
LRR + Cell + 9C + H2O2ndndnd0.8
LRR + Cell + 9Cndndnd0.8
  1. The microbial inoculum was collected from a food-waste-and-cow-manure fed reactor. Enzymes were supplied once at time 0 at 4 mg of protein/g of substrate, with and without following addition of H2O2. Hydrogen peroxide was supplied at 0 h and 24 h (1 min before sampling at vigorous stirring) at 0.1 mM final concentration. Deionized water was added in all reactions without H2O2. nd not detected
  2. aOnly headspace was sparged with N2
  3. bChromatograms are shown in Additional file 1: Figure S6
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Biotechnology for Biofuels

ISSN: 1754-6834

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