Fig. 4
From: A simple strategy to effectively produce d-lactate in crude glycerol-utilizing Escherichia coli
Effective production of d-lactate by the two-dose fermentation. Refer to Fig. 3 for the fermentation process. The fermentation was initiated with the cell density at OD550 of 0.2. The absorbance measurement of the cell density in the production phase was interfered with crude glycerol and Ca(OH)2 and, therefore, not reported. The experiment was duplicated. The typical fermentation profiles were reported for strain EcoB-170 (a) grown on 2 g/L yeast extract and fed with 80 g/L crude glycerol and (b) grown on 1 g/L yeast extract and fed with 90 g/L crude glycerol. Cell density (black circle); crude glycerol (black up-pointing triangle); d-lactate (black square)