Fig. 1

Short-term H₂ photoproduction under 1-s light/9-s dark pulse illumination protocol in C. reinhardtii. The CC-4533 and the flv 208 mutant cells were grown for 2 days at 50 µmol photons m⁻² s⁻¹ in TP medium bubbling with 3% CO₂, transferred to a vial equipped with H₂ and O₂ sensors, flushed with Ar. The intensity of light pulses was around 250 µmol photons m⁻² s⁻¹. a H₂ and O₂ yields during 10-min dark anaerobic adaptation phase, 20-min H₂ photoproduction phase and 3-min dark H₂ uptake phase. b Dark respiration rate monitored just before induction of the H₂ photoproduction. Experiments have been performed in 4 independent replicates, and exemplary measurements are presented in a. The values in b are the mean of all replicates (± SD). Statistical significance level: ***p < 0.001