Fig. 5
From: Elimination of the flavodiiron electron sink facilitates long-term H2 photoproduction in green algae
Effect of periodic 24-h growth recovery phases on long-term H2 photoproduction by the 1/9 pulse illumination protocol. C. reinhardtii CC-4533 and the flv 208 mutant were grown in TP medium for 2 days under 50 µmol photons m−2 s−1 bubbling with 3% CO2 before shifting to the H2 photoproduction conditions (Ar atmosphere and pulse illumination with 250 µmol photons m−2 s−1 pulses). The recovery phase includes 1:1 dilution with fresh TP medium and 24-h growth under 50 µmol photons m−2 s−1 bubbling with 3% CO2. a H2 photoproduction during 18 days. b Chl amount of the cultures during H2 photoproduction and recovery phases. c H2 production rates [µmol (mg Chl h)−1] during each cycle. These rates do not represent maximal H2 production rates as given in Additional file 1: Figure S6a. Values are mean of three independent replicates (± SD)