Fig. 6From: Elimination of the flavodiiron electron sink facilitates long-term H2 photoproduction in green algaeLong-term H2 photoproduction using a CBB-cycle substrate limitation protocol in C. reinhardtii CC-4533 and the flv 208 mutant. H2 photoproduction was induced by a 4-h dark anaerobic induction phase followed with constant 320 µmol photons m−2 s−1 illumination. Chlorophyll concentration was adjusted to ~ 50 µg Chl ml−1. a, b The daily H2 and O2 production (a, b; µmol mg Chl−1) in acetate-free HS medium during a 4-day experiment. c, d The effects of the iron-salt-based O2 absorbent on the H2 and O2 accumulation. e H2 production rates [µmol (mg Chl h)−1] during 96 h. Values are mean of 5 independent replicates (± SEM). Statistical significance levels: *p < 0.05; **p < 0.01; ***p < 0.001Back to article page