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Table 1 Specific activity and kinetic parameters of XylE and its hybrid enzymes

From: Improvement in catalytic activity and thermostability of a GH10 xylanase and its synergistic degradation of biomass with cellulase

EnzymesKm (mg/mL)Vmax (µmol/min/mg)kcat (/s)kcat/Km (mL/s/mg)Specific activity (U/mg)
XYL10C0.54 ± 0.023600 ± 662400 ± 164400 ± 1313200 ± 101
XylE0.75 ± 0.04680 ± 31430 ± 12570 ± 18610 ± 18
XylE-M30.61 ± 0.031390 ± 41860 ± 161340 ± 221310 ± 45
XylE-M60.42 ± 0.011160 ± 17740 ± 201760 ± 281150 ± 55
XylE-M90.46 ± 0.021390 ± 38900 ± 101960 ± 311130 ± 38
XylE-M3/M60.62 ± 0.022200 ± 281410 ± 222280 ± 292010 ± 51
XylE-M3/M90.42 ± 0.02880 ± 21570 ± 161370 ± 22730 ± 21
XylE-M6/M90.57 ± 0.011280 ± 34820 ± 191440 ± 181270 ± 37
XylE-M3/M6/M90.67 ± 0.031760 ± 471170 ± 261750 ± 191500 ± 46
XYL10C-M3NDNDNDNDND
XYL10C-M62.02 ± 0.042530 ± 771890 ± 56940 ± 231830 ± 66
XYL10C-M9NDNDNDNDND
  1. The specific activities mentioned were calculated after conducting assays with the enzymes at 70 °C using beechwood xylan as the substrate
  2. ND no activity detected