Improvement in catalytic activity and thermostability of a GH10 xylanase and its synergistic degradation of biomass with cellulase

Table 1 Specific activity and kinetic parameters of XylE and its hybrid enzymes

Enzymes	K_m (mg/mL)	V_max (µmol/min/mg)	k_cat (/s)	k_cat/K_m (mL/s/mg)	Specific activity (U/mg)
XYL10C	0.54 ± 0.02	3600 ± 66	2400 ± 16	4400 ± 131	3200 ± 101
XylE	0.75 ± 0.04	680 ± 31	430 ± 12	570 ± 18	610 ± 18
XylE-M3	0.61 ± 0.03	1390 ± 41	860 ± 16	1340 ± 22	1310 ± 45
XylE-M6	0.42 ± 0.01	1160 ± 17	740 ± 20	1760 ± 28	1150 ± 55
XylE-M9	0.46 ± 0.02	1390 ± 38	900 ± 10	1960 ± 31	1130 ± 38
XylE-M3/M6	0.62 ± 0.02	2200 ± 28	1410 ± 22	2280 ± 29	2010 ± 51
XylE-M3/M9	0.42 ± 0.02	880 ± 21	570 ± 16	1370 ± 22	730 ± 21
XylE-M6/M9	0.57 ± 0.01	1280 ± 34	820 ± 19	1440 ± 18	1270 ± 37
XylE-M3/M6/M9	0.67 ± 0.03	1760 ± 47	1170 ± 26	1750 ± 19	1500 ± 46
XYL10C-M₃	ND	ND	ND	ND	ND
XYL10C-M₆	2.02 ± 0.04	2530 ± 77	1890 ± 56	940 ± 23	1830 ± 66
XYL10C-M₉	ND	ND	ND	ND	ND

The specific activities mentioned were calculated after conducting assays with the enzymes at 70 °C using beechwood xylan as the substrate
ND no activity detected

ISSN: 2731-3654