Fig. 2

Hydrocarbon production using the 7336AAR double mutants. All mutants contained the S298A mutation in addition to the mutation described at the bottom. a, b Relative amount of total hydrocarbon (a) and pentadecane (b) produced in E. coli coexpressing 73102ADO and a double mutant of 7336AAR. The data are shown in descending order of the relative amount of total hydrocarbon. c Relative activity of AAR. The activities of C35S, T96S, A18S, M267V, and K198Q could not be obtained because the yields of soluble protein were too low to be detected by western blotting. d Fractions of pentadecane, heptadecene, and heptadecane relative to the total hydrocarbon yield. e Relative amount of soluble AAR in E. coli. f Relative amount of insoluble AAR in E. coli. g Solubility of AAR. h Relative protein expression levels of AAR. In all panels, a horizontal dotted line shows the value for the S298A single mutant, which was used as a control (denoted by * and “S298A (Control)”). In panels a–c, e, f, and h, the values are normalized to those of the S298A control. All measurements were taken in triplicate, and the mean ± standard error is shown