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Fig. 4 | Biotechnology for Biofuels

Fig. 4

From: Improving hydrocarbon production by engineering cyanobacterial acyl-(acyl carrier protein) reductase

Fig. 4

Hydrocarbon production using the 7336AAR multiple mutants. a, b Relative amount of total hydrocarbon (a) and pentadecane (b) produced in E. coli coexpressing 73102ADO and a multiple mutant of 7336AAR. c Relative activity of AAR. d Fractions of pentadecane, heptadecene, and heptadecane relative to the total amount of hydrocarbon. e Relative amount of soluble AAR in E. coli. f Relative amount of insoluble AAR in E. coli. g Solubility of AAR. h Relative protein expression level of AAR. In all panels, a horizontal dotted line shows the value for the S298A single mutant, which was used as a control (denoted by * and “298 (Control)”), and 13, 20, 60, 110, 200, and 298 denote the N13Q, L20R, V60I, K110D, S200E, and S298A mutations, respectively. In panels ac, e, f, and h, the values are normalized to those of the S298A control. All measurements were taken in triplicate, and the mean ± standard error is shown

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