Fig. 5

Hydrocarbon production using the 7336AAR multiple mutants with a weakened T7 promoter. a, b Relative amount of total hydrocarbon (a) and relative activity (b). Gray (a) and dark green bars (b) show the relative amount of total hydrocarbon and the relative activity, respectively, obtained using a weakened T7 promoter to decrease protein expression levels. White (a) and light green bars (b) show the relative amount of total hydrocarbon and the relative activity measured using the wild-type T7 promoter, which are the same as those shown in Fig. 4a, c, respectively. Horizontal dotted lines show the values for the S298A single mutant, which was used as a control (denoted by * and “298 (Control)”)