Table 2 Absolute amounts of hydrocarbons produced in E. coli coexpressing 73102ADO and AAR using M9-rich medium
From: Improving hydrocarbon production by engineering cyanobacterial acyl-(acyl carrier protein) reductase
AAR | Mutations | Total hydrocarbons (mg/L) | Pentadecane (C15:0) (mg/L) | Heptadecene (C17:1) (mg/L) | Heptadecane (C17:0) (mg/L) |
|---|---|---|---|---|---|
7336AAR | 13/60/110/200/298 | 28.0 ± 1.5 | 11.0 ± 0.5 | 16.7 ± 1.0 | 0.28 ± 0.01 |
7336AAR | 13/110/200/298 | 27.5 ± 1.9 | 13.1 ± 1.0 | 14.1 ± 0.9 | 0.35 ± 0.04 |
7336AAR | 13/60/110/298 | 29.1 ± 1.5 | 14.6 ± 0.8 | 14.2 ± 0.7 | 0.39 ± 0.02 |
7336AAR | S298A | 6.8 ± 0.3 | 4.3 ± 0.2 | 2.3 ± 0.1 | 0.13 ± 0.01 |
7942AAR | Wild-type | 26.5 ± 0.2 | 7.7 ± 0.1 | 18.6 ± 0.1 | 0.27 ± 0.01 |