Table 1 Kinetic constants of NcLPMO9C for H2O2 and hydrocoerulignone determined in 50 mM sodium phosphate buffer, pH 6.0
From: Improved spectrophotometric assay for lytic polysaccharide monooxygenase
H2O2 catalytic constants | Hydrocoerulignone catalytic constants | ||||
|---|---|---|---|---|---|
Hydrocoerulignone (µM) | KM,app (µM) | Vmax,app (U g−1) | H2O2 (µM) | KM,app (µM) | Vmax,app (U g−1) |
2000 | 7.8 ± 0.8 | 194.2 ± 4.2 | 300 | 4800 ± 900 | 644 ± 93 |
1000 | 4.3 ± 0.6 | 118.1 ± 2.8 | 100 | 3600 ± 500 | 534 ± 58 |
500 | 4.2 ± 0.5 | 63.5 ± 1.2 | 50 | 2100 ± 200 | 327 ± 21 |
250 | 2.7 ± 0.3 | 36.4 ± 0.7 | 25 | 1700 ± 300 | 255 ± 28 |
125 | 1.7 ± 0.5 | 19.1 ± 0.6 | 12.5 | 2000 ± 400 | 250 ± 29 |
62.5 | 1.4 ± 0.2 | 12.5 ± 0.2 | 6.25 | 1300 ± 400 | 151 ± 22 |
3.18 | 600 ± 100 | 71 ± 6 | |||