Improved spectrophotometric assay for lytic polysaccharide monooxygenase

Table 2 Specific activities of six different LPMOs from two different organisms

	Specific activity (U g⁻¹)
	Hydrocoerulignone	2,6-DMP
Neurospora crassa
LPMO9C	138 ± 12	31 ± 3
LPMO9E	113 ± 4	22 ± 3
LPMO9J	86 ± 4	13 ± 1
Crassicarpon hotsonii (syn. Myriococcum thermophilum)
LPMO (gene identifier) Myrth2p4_000359	120 ± 5	30 ± 1
LPMO (gene identifier) Myrth2p4_004260	137 ± 4	16 ± 1
LPMO (gene identifier) Myrth2p4_006403	110 ± 2	38 ± 2

Measured under standard conditions with 500 µM hydrocoerulignone (100 mM sodium acetate buffer, pH 6.0 containing 100 µM H₂O₂) and 2,6-DMP (50 mM sodium phosphate buffer, pH 7.5 containing 100 µM H₂O₂). Data expressed as mean values (± SD), from three independent repeats

ISSN: 2731-3654