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Table 3 Effect of media components on NcLPMO9C activity

From: Improved spectrophotometric assay for lytic polysaccharide monooxygenase

 

Residual activity (%)

Hydrocoerulignone

2,6-DMP

Concentration (g l−1)

1.0

10.0

1.0

10.0

Yeast nitrogen base (YNB)a

78.8 ± 3.2

29.0 ± 3.9

85.3 ± 3.9

40.1 ± 3.7

Meat peptone

52.6 ± 1.3

23.2 ± 1.5

68.5 ± 5.0

10.9 ± 2.4

Casein peptone

51.8 ± 3.3

12.8 ± 1.0

62.6 ± 2.3

15.6 ± 1.9

Yeast extract

29.9 ± 1.5

13.8 ± 1.5

50.9 ± 1.8

4.4 ± 0.8

  1. Residual activities were calculated based on the concentration of added NcLPMO9C and its determined specific activity (138 U g−1 with hydrocoerulignone; 23 U g−1 2,6-DMP). Assay conditions for hydrocoerulignone or 2,6-DMP respectively: 500 or 2000 µM hydrocoerulignone or 2,6-DMP, 0.3 or 2.0 µM NcLPMO9C, 100 µM H2O2 in 100 mM sodium acetate buffer at pH 6.0 and 30 °C. The pH was measured before and after measurements. Data expressed as mean values (± SD), from three independent repeats
  2. aYNB 9 g l−1
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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