Table 4 Effect of amino acids on NcLPMO9C activity
From: Improved spectrophotometric assay for lytic polysaccharide monooxygenase
Residual activity (%) | ||
|---|---|---|
Hydrocoerulignone | 2,6-DMP | |
Arg (R) | 43.9 ± 0.7 | 34.0 ± 3.1 |
His (H) | 0.3 ± 0.3 | 0.1 ± 0.1 |
Lys (K) | 53.5 ± 2.7 | 36.0 ± 3.7 |
Asp (D)a | 39.9 ± 3.3 | 50.8 ± 1.5 |
Glu (E)a | 57.4 ± 2.0 | 46.5 ± 4.8 |
Ser (S) | 19.1 ± 0.9 | 34.6 ± 1.2 |
Thr (T) | 13.0 ± 0.5 | 18.5 ± 1.6 |
Asn (N) | 10.8 ± 0.4 | 10.6 ± 0.7 |
Gln (Q) | 25.2 ± 0.9 | 35.9 ± 1.6 |
Cys (C) | 3.7 ± 0.2 | 0.1 ± 0.1 |
Gly (G) | 49.1 ± 1.2 | 44.0 ± 3.4 |
Pro (P) | 48.5 ± 1.6 | 37.1 ± 1.1 |
Ala (A) | 39.9 ± 1.7 | 62.5 ± 0.3 |
Val (V) | 23.3 ± 2.3 | 42.4 ± 2.2 |
Ile (I) | 35.5 ± 0.9 | 47.9 ± 3.2 |
Leu (L) | 38.6 ± 1.1 | 49.1 ± 0.8 |
Met (M) | 12.0 ± 0.6 | 21.7 ± 1.7 |
Phe (F) | 7.5 ± 0.4 | 14.9 ± 0.1 |
Tyr (Y)a | 81.5 ± 3.6 | 91.8 ± 8.3 |
Trp (W)a | 3.5 ± 0.4 | 16.2 ± 2.3 |