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Table 3 Estimation of kcat/Km and product profile of the processive cellulases on cello-oligosaccharides with DP 5–8

From: Valorization of waste forest biomass toward the production of cello-oligosaccharides with potential prebiotic activity by utilizing customized enzyme cocktails

Enzyme namekcat/Km (min−1 M−1)Mode of actiona
C5C6C7C8C5C6C7C8
1. CcCel48A1.37 × 1032.89 × 1034.35 × 1034.41 × 103C3 + C2C4 to C2C4 to C1C5 to C1
2. CtCbh5A8.18 × 1038.15 × 1038.3 × 1039.01 × 103C3 + C2C4 to C2C4 to C2C6 to C1
3. CtCel9A6.45 × 1026.76 × 1022.55 × 1029.3 × 101C3 + C2C4 to C2C4 to C2C5 to C2
4. CtCbh9A7.46 × 1027.61 × 1022.01 × 1022.36 × 102C4 to C1C4 to C2C4 to C2C5 to C2
5. CtCel9B1.71 × 1031.56 × 1031.59 × 1031.88 × 103C3 + C2C4 to C2C5 to C2C6 to C2
6. PaCbh6A5.13 × 1033.46 × 1048.87 × 1048.11 × 104C3 + C2C4 to C2C4 to C2C5 to C2
7. CcCel9W1.13 × 1038.74 × 1032.18 × 1042.86 × 104C3 + C2C4 to C2C4 to C2C5 to C2
8. CcCel9M2.99 × 1042.52 × 1043.17 × 1042.78 × 104C4 + C1C4 + C2C4 to C2C5 to C2
9. CcCel9R2.04 × 1031.23 × 1038.51 × 102C3C4 + C3C5 to C3
10. CcCel9A1.37 × 1031.34 × 1031.34 × 1031.79 × 103C4 to C1C4 to C2C5 to C2C4 to C2
11. CsCbh48A1.00 × 1031.56 × 1032.97 × 1033.70 × 103C3 + C2C4 to C2C6 to C1C6 to C1
12. CcCel9J
13. CcCel9Q3.26 × 1033.81 × 1035.04 × 1036.51 × 103C3 + C2C4 to C2C4 to C2C6 to C2
14. RfCel9A1.12 × 1021.94 × 1035.77 × 1035.93 × 103C4 to C2C5 to C2C6 to C2
  1. aC2 + C3” represents the products C2 and C3, while “C4 to C1” represents a range of products with degree of polymerization varying between 4 and 1, i.e., from cellotetraose to glucose (C4 + C3 + C2 + C1)