Fig. 2
Characterization of stable transgenic P32 and P605 calli expressing pporRFP fluorescent fusion protein. a–h Micrographs of 1-month-old pporRFP transgenic and non-transgenic (Agrobacterium GV3101 harboring no construct) in clones P32 and P605 callus. a–d Bright-field images of P32 (a, b) and P605 calli (c, d). e–h PporRFP fluorescence images of P32 (e, f) and P605 (g, h) calli. a, e Transgenic P32 calli. b, f, Non-transgenic P32 calli. c and g Transgenic P605 calli. d, h Non-transgenic P605 calli. i Graph of pporRFP fluorescence intensity measurements plotted as count per second (cps × 105). Ten independent stable transgenic P32 (gray columns) and P605 (black columns) calli were used. Each column represents the average fluorescence intensity measured from three independent callus pieces (n = 3 for each line) at the pporRFP peak emission wavelength (591 nm). All fluorescent measurements were normalized to the non-transgenic calli control. Error bars represent the mean ± SE of three biological replicates, and different letters denote a statistically significant difference among means at a p value < 0.05 according to one-way ANOVA (Tukey’s test). Bars = 2 mm in a–h