Fig. 4
Characterization of regenerated T0 P32 and P605 plants expressing pporRFP. a–p Stereomicroscope images showing the presence of pporRFP fluorescence signal in 3-month-old transgenic and non-transgenic P32 and P605 plants regenerated from stable transgenic and non-transgenic single-cell suspension cultures. a–d Bright-field images of P32 leaves/stems (a, b) and roots (c, d). e–h PporRFP fluorescent images of P32 leaves/stems (e, f) and roots (g, h). i–l White light images of P605 leaves/stems (i, j) and roots (k, l). m–p PporRFP fluorescent images of P605 leaves/stems (m, n) and roots (o, p). q Graph of pporRFP fluorescence intensity measurements plotted as count per second (cps × 105) of 10 independent transgenic P32 (gray columns) and P605 (gray columns) plants. Fluorescence intensity was measured from youngest fully developed leaves of 10 individual T0 plants of each line. Each column (n = 3 leaves) represents the average fluorescence intensity at the peak emission wavelength of pporRFP (591 nm). All fluorescent measurements were normalized to the non-transgenic control plants. r Expression of reporter gene pporRFP in leaves, stems and roots of T0 transgenic P32 (gray columns) and P605 (black columns) as revealed by qRT-PCR. Error bars represent the mean ± SE of three biological replicates, and different letters denote a statistically significant difference among means at a p value < 0.05 according to one-way ANOVA (Tukey’s test). Bars = 0.5 cm. in a–p