Skip to main content

Table 1 Transformation efficiencies of Agrobacterium tumefaciens-mediated transformation of P32 and P605 cell suspension cultures

From: Embryogenic cell suspensions for high-capacity genetic transformation and regeneration of switchgrass (Panicum virgatum L.)

Bacterial strainSelection geneReporter geneP32P605
Total no. of calli grownTotal no. of fluorescent calliPercentage efficiency (%)Total no. of calli grownTotal no. of fluorescent calliPercentage efficiency (%)
GV3101HYGRpporRFP+1900 ± 0.071225 ± 1.7868.47 ± 3.78b2128 ± 1.381520 ± 0.2884.42 ± 2.48a
GV2260HYGRpporRFP+1503 ± 0.03820 ± 1.6654.66 ± 5.66b1977 ± 0.331040 ± 0.6757.63 ± 4.47b
EHA105HYGRpporRFP+1338 ± 0.6435 ± 2.8930.76 ± 2.89c1454 ± 0.86623 ± 0.7642.85 ± 2.58c
GV3850HYGRpporRFP+05.00 ± 0000.00 ± 000.00 ± 00d390 ± 0.17156 ± 1.762.5 ± 2.29d
  1. Transformation efficiency was calculated for lines of P32 and P605 cell suspension cultures using Agrobacterium strains GV3101, GV2260, EHA105 and GV3850 (OD600 = 0.5). Transformation efficiency was evaluated by scoring growing hygromycin B (HYG R)-resistant calli and expressing the fluorescent pporRFP protein. Data are mean ± SD of three replications of transformation events (n = 10 plates scored per transformation event for each A. tumefaciens strain and per each line). Different letters denote statistically significant differences among means at a p value < 0.05 according to one-way ANOVA (Tukey’s test)