Embryogenic cell suspensions for high-capacity genetic transformation and regeneration of switchgrass (Panicum virgatum L.)

Table 1 Transformation efficiencies of Agrobacterium tumefaciens-mediated transformation of P32 and P605 cell suspension cultures

Bacterial strain	Selection gene	Reporter gene	P32			P605
Bacterial strain	Selection gene	Reporter gene	Total no. of calli grown	Total no. of fluorescent calli	Percentage efficiency (%)	Total no. of calli grown	Total no. of fluorescent calli	Percentage efficiency (%)
GV3101	HYG^R	pporRFP+	1900 ± 0.07	1225 ± 1.78	68.47 ± 3.78^b	2128 ± 1.38	1520 ± 0.28	84.42 ± 2.48^a
GV2260	HYG^R	pporRFP+	1503 ± 0.03	820 ± 1.66	54.66 ± 5.66^b	1977 ± 0.33	1040 ± 0.67	57.63 ± 4.47^b
EHA105	HYG^R	pporRFP+	1338 ± 0.6	435 ± 2.89	30.76 ± 2.89^c	1454 ± 0.86	623 ± 0.76	42.85 ± 2.58^c
GV3850	HYG^R	pporRFP+	05.00 ± 00	00.00 ± 00	0.00 ± 00^d	390 ± 0.17	156 ± 1.76	2.5 ± 2.29^d

Transformation efficiency was calculated for lines of P32 and P605 cell suspension cultures using Agrobacterium strains GV3101, GV2260, EHA105 and GV3850 (OD₆₀₀ = 0.5). Transformation efficiency was evaluated by scoring growing hygromycin B (HYG ^R)-resistant calli and expressing the fluorescent pporRFP protein. Data are mean ± SD of three replications of transformation events (n = 10 plates scored per transformation event for each A. tumefaciens strain and per each line). Different letters denote statistically significant differences among means at a p value < 0.05 according to one-way ANOVA (Tukey’s test)

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