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Table 2 Frequencies of shooting, rooting, and viable plant regeneration (mean ± SD) from three replicated experiments starting from 100 micro-calli per line

From: Embryogenic cell suspensions for high-capacity genetic transformation and regeneration of switchgrass (Panicum virgatum L.)

 

Shooting efficiency (%)

Rooting efficiency (%)

Plants regenerated (%)

Regeneration efficiencies of plantlets from calli

 Transgenic P32

73.75 ± 2.92cd

69.15 ± 1.35de

81 ± 4.88bc

 Non-transgenic P32

79 ± 3.51bc

74.05 ± 2.35cd

85.5 ± 1.42b

 Transgenic P605

62.25 ± 1.15e

58.75 ± 0.76e

60.25 ± 1.73de

 Non-transgenic P605

71.67 ± 1.73cd

66.25 ± 2.41de

75.13 ± 2.00c

Regeneration efficiencies of plantlets from cell suspension cultures

 Transgenic P32

100 ± 00a

91.5 ± 2.11a

100 ± 00a

 Non-transgenic P32

100 ± 00a

93.5 ± 3.75a

99.23 ± 0.43a

 Transgenic P605

100 ± 00a

85 ± 2.89b

95.28 ± 1.86a

 Non-transgenic P605

100 ± 00a

88.5 ± 0.82b

97.78 ± 1.30a

  1. After shooting, the rooting percentage represents the frequency of shoots that produced root systems. After rooting, the percentage of regenerated plants represents the frequency of the rooted shoots that survived to make viable plants in pots. Different letters denote a statistically significant difference among means at a p value < 0.05 according to a 2-way ANOVA (Tukey’s test)