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Fig. 5 | Biotechnology for Biofuels

Fig. 5

From: Upgrading of efficient and scalable CRISPR–Cas-mediated technology for genetic engineering in thermophilic fungus Myceliophthora thermophila

Fig. 5

CRISPR–Cas12a-assisted marker recycling editing of eleven target genes, including nine genes involved in the cellulase production pathway, via three successive round transformations. The 11 genes were cre-1, res-1, gh1-1, alp-1, xyr-1, rca-1, hcr-1, ap-3, prk-6, and two selectable marker genes neo and bar. HDR, homology-directed repair; WT, wild type; DR, 19 nt direct repeat; U6p, U6 promoter; Ptef1, tef1 promoter; TtrpC, trpC terminator. M, mutant strain. PtrpC, trpC promoter. TAA, stop codon. TTTTTT, poly-T sequence

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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