Fig. 4

Effects of enzyme supplementation modes on the release of glucose, xylose, and TRS during the fed-batch enzymatic hydrolysis. The fed-batch hydrolysis was initiated at 12% (w/v) solids loading, and 7% fresh substrate was fed four times (3, 6, 24, and 48 h) to reach 40% (w/v) solids loading. The enzyme supplementation modes were as follows: a The enzyme blend (based on the final solids loading, 40%, w/v) was added completely at the beginning of the hydrolysis (hollow symbols). b The enzyme blend was fed simultaneously with the pretreated corn stover at each time point of feeding to maintain an identical final enzyme dosage (solid symbols)