Fig. 5

Corn stover to fermentable sugars mass balance analysis. The analysis was based on the fed-batch enzymatic hydrolysis of the SMs-pretreated corn stover. The hydrolysis was initiated at 12% (w/v) solids loading, and 7% substrate was fed four times (3, 6. 24, and 48 h) to reach a final solids loading of 40% (w/v). The enzyme cocktail was fed simultaneously with the substrate at each feeding time point. The hydrolysis was held at 50 °C, pH 4.8, and 200 rpm for 120 h