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Table 2 Results of fed-batch enzymatic hydrolysis with different feeding times and the chemical compositions of the solid residues after enzymatic hydrolysis

From: Fed-batch enzymatic hydrolysis of alkaline organosolv-pretreated corn stover facilitating high concentrations and yields of fermentable sugars for microbial lipid production

Entry

Solids loading (%, w/v)

Feeding times (time point of feeding, h)b

Hydrolysates

Solid residues after enzymatic hydrolysis

Sugar concentration (g/L)

Composition (%, w/w)

Glucose

Cello-oligosaccharides

Xylose

Xylo-oligosaccharides

TRS

Glucan

Xylan

Total lignin

Ash

1

12

54.0 (± 1.0)

1.7 (± 0.5)

23.8 (± 0.3)

6.0 (± 0.3)

94.3 (± 2.7)

14.4 (± 0.3)

7.8 (± 0.4)

37.6 (± 1.0)

29.5 (± 0.2)

2

19

1 (3)

81.7 (± 1.2)

3.0 (± 0.5)

33.6 (± 1.3)

11.6 (± 0.7)

138.4 (± 4.0)

17.1 (± 0.4)

8.5 (± 0.1)

34.4 (± 1.2)

28.6 (± 0.7)

3

26

2 (3–6)

102.0 (± 3.0)

8.7 (± 1.1)

42.9 (± 1.3)

15.6 (± 1.1)

183.6 (± 3.8)

18.4 (± 0.2)

8.8 (± 0.2)

32.4 (± 0.8)

25.7 (± 1.2)

4

33

3 (3–6–24)

121.0 (± 1.7)

13.9 (± 2.1)

50.0 (± 0.9)

22.5 (± 1.9)

213.6 (± 2.0)

21.0 (± 0.8)

9.7 (± 0.4)

30.8 (± 1.4)

18.1 (± 0.2)

5

40

4 (3–6–24–48)

135.0 (± 0.0)

17.9 (± 1.0)

56.0 (± 0.5)

27.9 (± 1.3)

235.5 (± 3.0)

26.2 (± 0.4)

10.8 (± 0.2)

27.2 (± 0.4)

16.9 (± 0.6)

6a

40

4 (3–6–24–48)

146.7 (± 9.2)

15.6 (± 2.0)

58.7 (± 3.6)

25.9 (± 2.6)

251.9 (± 10.2)

21.7 (± 0.5)

9.4 (± 0.3)

30.7 (± 0.1)

18.3 (± 0.3)

  1. aThe enzyme blend was fed four times simultaneously with the substrate
  2. bThe fed-batch enzymatic hydrolysis was initiated at 12% (w/v) solids loading and 7% (w/v) substrate was fed different times