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Table 2 Results of fed-batch enzymatic hydrolysis with different feeding times and the chemical compositions of the solid residues after enzymatic hydrolysis

From: Fed-batch enzymatic hydrolysis of alkaline organosolv-pretreated corn stover facilitating high concentrations and yields of fermentable sugars for microbial lipid production

Entry Solids loading (%, w/v) Feeding times (time point of feeding, h)b Hydrolysates Solid residues after enzymatic hydrolysis
Sugar concentration (g/L) Composition (%, w/w)
Glucose Cello-oligosaccharides Xylose Xylo-oligosaccharides TRS Glucan Xylan Total lignin Ash
1 12 54.0 (± 1.0) 1.7 (± 0.5) 23.8 (± 0.3) 6.0 (± 0.3) 94.3 (± 2.7) 14.4 (± 0.3) 7.8 (± 0.4) 37.6 (± 1.0) 29.5 (± 0.2)
2 19 1 (3) 81.7 (± 1.2) 3.0 (± 0.5) 33.6 (± 1.3) 11.6 (± 0.7) 138.4 (± 4.0) 17.1 (± 0.4) 8.5 (± 0.1) 34.4 (± 1.2) 28.6 (± 0.7)
3 26 2 (3–6) 102.0 (± 3.0) 8.7 (± 1.1) 42.9 (± 1.3) 15.6 (± 1.1) 183.6 (± 3.8) 18.4 (± 0.2) 8.8 (± 0.2) 32.4 (± 0.8) 25.7 (± 1.2)
4 33 3 (3–6–24) 121.0 (± 1.7) 13.9 (± 2.1) 50.0 (± 0.9) 22.5 (± 1.9) 213.6 (± 2.0) 21.0 (± 0.8) 9.7 (± 0.4) 30.8 (± 1.4) 18.1 (± 0.2)
5 40 4 (3–6–24–48) 135.0 (± 0.0) 17.9 (± 1.0) 56.0 (± 0.5) 27.9 (± 1.3) 235.5 (± 3.0) 26.2 (± 0.4) 10.8 (± 0.2) 27.2 (± 0.4) 16.9 (± 0.6)
6a 40 4 (3–6–24–48) 146.7 (± 9.2) 15.6 (± 2.0) 58.7 (± 3.6) 25.9 (± 2.6) 251.9 (± 10.2) 21.7 (± 0.5) 9.4 (± 0.3) 30.7 (± 0.1) 18.3 (± 0.3)
  1. aThe enzyme blend was fed four times simultaneously with the substrate
  2. bThe fed-batch enzymatic hydrolysis was initiated at 12% (w/v) solids loading and 7% (w/v) substrate was fed different times