Table 2 Results of fed-batch enzymatic hydrolysis with different feeding times and the chemical compositions of the solid residues after enzymatic hydrolysis
Entry | Solids loading (%, w/v) | Feeding times (time point of feeding, h)b | Hydrolysates | Solid residues after enzymatic hydrolysis | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
Sugar concentration (g/L) | Composition (%, w/w) | ||||||||||
Glucose | Cello-oligosaccharides | Xylose | Xylo-oligosaccharides | TRS | Glucan | Xylan | Total lignin | Ash | |||
1 | 12 | – | 54.0 (± 1.0) | 1.7 (± 0.5) | 23.8 (± 0.3) | 6.0 (± 0.3) | 94.3 (± 2.7) | 14.4 (± 0.3) | 7.8 (± 0.4) | 37.6 (± 1.0) | 29.5 (± 0.2) |
2 | 19 | 1 (3) | 81.7 (± 1.2) | 3.0 (± 0.5) | 33.6 (± 1.3) | 11.6 (± 0.7) | 138.4 (± 4.0) | 17.1 (± 0.4) | 8.5 (± 0.1) | 34.4 (± 1.2) | 28.6 (± 0.7) |
3 | 26 | 2 (3–6) | 102.0 (± 3.0) | 8.7 (± 1.1) | 42.9 (± 1.3) | 15.6 (± 1.1) | 183.6 (± 3.8) | 18.4 (± 0.2) | 8.8 (± 0.2) | 32.4 (± 0.8) | 25.7 (± 1.2) |
4 | 33 | 3 (3–6–24) | 121.0 (± 1.7) | 13.9 (± 2.1) | 50.0 (± 0.9) | 22.5 (± 1.9) | 213.6 (± 2.0) | 21.0 (± 0.8) | 9.7 (± 0.4) | 30.8 (± 1.4) | 18.1 (± 0.2) |
5 | 40 | 4 (3–6–24–48) | 135.0 (± 0.0) | 17.9 (± 1.0) | 56.0 (± 0.5) | 27.9 (± 1.3) | 235.5 (± 3.0) | 26.2 (± 0.4) | 10.8 (± 0.2) | 27.2 (± 0.4) | 16.9 (± 0.6) |
6a | 40 | 4 (3–6–24–48) | 146.7 (± 9.2) | 15.6 (± 2.0) | 58.7 (± 3.6) | 25.9 (± 2.6) | 251.9 (± 10.2) | 21.7 (± 0.5) | 9.4 (± 0.3) | 30.7 (± 0.1) | 18.3 (± 0.3) |