Fig. 1From: Screening for functional IRESes using α-complementation system of β-galactosidase in Pichia pastorisBackground activity of LacZ gene in P. pastoris. a Schematic diagram of expression vectors with or without AOX1 promoter. b Analysis of enzymatic activity of β-galactosidase on BMMY plates containing X-Gal. The vector pPICZA-LacZ that the transcription of LacZ gene is driven by AOX1 promoter was used as positive control, and empty vector pPICZA was used as negative control. c Quantitative analysis of enzymatic activity of β-galactosidaseBack to article page