Fig. 2
From: Screening for functional IRESes using α-complementation system of β-galactosidase in Pichia pastoris
Development of the α-complementation of β-galactosidase in P. pastoris. a Schematic diagram of vectors containing α-peptides and ω-peptides with different lengths. b Detection of β-galactosidase activity on plates containing X-gal. Empty vector pAO815 and pPICZA as negative controls; full-length LacZ gene (LacZ WT) as a positive control. c The relative activities of β-galactosidase in the α-complementation systems with different lengths of α-peptide and ω-peptide. Normalization was performed with full-length β-galactosidase (LacZ WT) activity at 100%