Fig. 5From: Screening for functional IRESes using α-complementation system of β-galactosidase in Pichia pastorisDetection of cryptic splicing in bicistronic mRNA by RT-PCR. a Schematic diagram of cryptic splicing and primer design. b Detection of RT-PCR products on agarose gel. NC, PCR negative control reaction without template. ∓, reactions without or with reverse transcriptase, respectivelyBack to article page