Fig.Ā 2From: Structure-based directed evolution improves S. cerevisiae growth on xylose by influencing in vivo enzyme performancePirXI V270AāA273G improves growth of S. cerevisiae strain DS75543 on xylose. The orange and green lines represent growth on xylose (20Ā gĀ lā1) of yeast expressing the mutant and the wild-type PirXI, respectively. a, b Growth in 96-well plate with continuous measurement of optical density. Each line represents an individual clone selected from a transformation plate. c Growth in triplicate in batch culture with intermittent sampling and measurement of optical densitiesBack to article page