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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: Structure-based directed evolution improves S. cerevisiae growth on xylose by influencing in vivo enzyme performance

Fig. 3

Expression level and cell-free extract activities of PirXI variants. Samples of 2 µg of crude extract protein prepared from cells grown on xylose medium were loaded on an SDS gel. The dominant bands at ~ 50 kDa represent PirXI. The expression level calculated by measuring the intensity of the bands is about 25% for both samples. WT: wild-type PirXI VA: V270A–A273G PirXI

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