Fig.Ā 5
Effect of metals on thermostability of PirXI. The graphs show the apparent melting temperatures of purified and EDTA-treated wild-type PirXI (green lines) and the V270AāA273G mutant PirXI (orange lines) in the presence of various concentrations of metals (a Mn2+; b Mg2+; and c Ca2+). The Tm values are averages from two independent measurements. [E]ā=ā20Ā Ī¼M. Error bars represent standard deviations