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Table 1 Design of PirXI library LibM1

From: Structure-based directed evolution improves S. cerevisiae growth on xylose by influencing in vivo enzyme performance

ResidueAmino acidsCodonsFreq. WTa
V270V A GGBT0.33
A273A GGST0.5
T274T I K QAYC + MAA0.25
W307W F Y I S T NWHT + TGG0.14
T309T S K R N QAVS + CAG0.29
  1. aCodons at each position were selected in such a way that each desired amino acid, including the wild type, is included at a frequency of 1/(total diversity)