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TableĀ 1 Design of PirXI library LibM1

From: Structure-based directed evolution improves S. cerevisiae growth on xylose by influencing in vivo enzyme performance

Residue

Amino acids

Codons

Freq. WTa

V270

V A G

GBT

0.33

A273

A G

GST

0.5

T274

T I K Q

AYCā€‰+ā€‰MAA

0.25

W307

W F Y I S T N

WHTā€‰+ā€‰TGG

0.14

T309

T S K R N Q

AVSā€‰+ā€‰CAG

0.29

  1. aCodons at each position were selected in such a way that each desired amino acid, including the wild type, is included at a frequency of 1/(total diversity)