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TableĀ 3 Library LibM2 variants and codons

From: Structure-based directed evolution improves S. cerevisiae growth on xylose by influencing in vivo enzyme performance

Residue

Variants

Codons

Freq. WT

S141

S I V A T G N

RBCā€‰+ā€‰AAC

0.14

T142

T S

ASC

0.5

A143

A T G S

RSC

0.25

N144

N L Q T

CWGā€‰+ā€‰AMC

0.25

V145

V L I C

VTTā€‰+ā€‰TGC

0.25

G147

G A T S

RSC

0.25