Enhanced lipid production by Yarrowia lipolytica cultured with synthetic and waste-derived high-content volatile fatty acids under alkaline conditions

Gao, Ruiling; Li, Zifu; Zhou, Xiaoqin; Bao, Wenjun; Cheng, Shikun; Zheng, Lei

doi:10.1186/s13068-019-1645-y

Biotechnology for Biofuels and Bioproducts

Table 1 Biomass and lipid production of Y. lipolytica with high concentrations of acetic acid as carbon source at different pH conditions

From: Enhanced lipid production by Yarrowia lipolytica cultured with synthetic and waste-derived high-content volatile fatty acids under alkaline conditions

Carbon source and initial pH	Biomass (g/L)	Lipid conc. (g/L)	Lipid content (wt%)	Y_X/S (g/g)	Y_L/S (g/g)	Lag phase (day)
30 g/L of acetic acid
pH = 6	10.56 ± 0.73	1.73 ± 0.08	16.41 ± 1.08	0.352 ± 0.024	0.058 ± 0.003	3–5
pH = 7	17.28 ± 0.85	5.16 ± 0.19	29.89 ± 0.89	0.576 ± 0.028	0.172 ± 0.006	0^a
pH = 8	17.86 ± 0.76	5.24 ± 0.23	29.33 ± 0.86	0.595 ± 0.025	0.175 ± 0.008	0
pH = 9	17.05 ± 0.61	4.57 ± 0.25	26.81 ± 1.07	0.568 ± 0.020	0.152 ± 0.008	0
pH = 10	13.67 ± 0.58	2.75 ± 0.11	20.14 ± 1.46	0.456 ± 0.019	0.092 ± 0.004	0
50 g/L of acetic acid
pH = 6	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
pH = 7	27.02 ± 1.21	7.40 ± 0.25	27.38 ± 0.83	0.540 ± 0.024	0.148 ± 0.005	0–1
pH = 8	28.92 ± 1.15	8.01 ± 0.30	27.69 ± 0.96	0.578 ± 0.023	0.160 ± 0.006	0
pH = 9	27.39 ± 1.22	7.10 ± 0.28	25.93 ± 1.35	0.548 ± 0.024	0.142 ± 0.006	0–1
pH = 10	17.07 ± 1.09	3.23 ± 0.09	18.95 ± 0.94	0.341 ± 0.022	0.065 ± 0.002	1–3
70 g/L of acetic acid
pH = 6	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
pH = 7	29.10 ± 1.45	6.76 ± 0.36	23.23 ± 1.04	0.497 ± 0.025	0.115 ± 0.006	1–3
pH = 8	37.14 ± 1.56	10.11 ± 0.42	27.22 ± 0.92	0.531 ± 0.022	0.144 ± 0.006	0
pH = 9	35.67 ± 1.64	9.09 ± 0.40	25.49 ± 1.11	0.510 ± 0.023	0.130 ± 0.006	2–3
pH = 10	13.35 ± 0.83	1.84 ± 0.13	13.75 ± 1.50	0.269 ± 0.017	0.037 ± 0.003	3–5
90 g/L of acetic acid
pH = 6	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
pH = 7	19.96 ± 0.88	4.36 ± 0.18	21.85 ± 1.01	0.291 ± 0.013	0.064 ± 0.003	1–3
pH = 8	31.02 ± 1.22	7.90 ± 0.37	25.47 ± 1.23	0.417 ± 0.016	0.106 ± 0.005	0–1
pH = 9	24.19 ± 1.19	5.92 ± 0.32	24.46 ± 1.40	0.319 ± 0.016	0.078 ± 0.004	3–4
pH = 10	9.606 ± 0.86	1.38 ± 0.06	14.32 ± 1.07	0.171 ± 0.015	0.024 ± 0.001	6–7
110 g/L of acetic acid
pH = 6	N.D.	N.D.	N.D.	N.D.	N.D.	N.D.
pH = 7	15.80 ± 0.93	2.49 ± 015	15.75 ± 0.89	0.192 ± 0.011	0.030 ± 0.002	4–5
pH = 8	17.33 ± 0.62	3.85 ± 0.19	22.21 ± 1.28	0.198 ± 0.007	0.044 ± 0.002	2
pH = 9	15.40 ± 0.77	3.30 ± 0.19	21.45 ± 1.53	0.177 ± 0.009	0.038 ± 0.002	3–5
pH = 10	10.20 ± 1.23	1.19 ± 0.10	11.67 ± 1.03	0.167 ± 0.020	0.019 ± 0.002	9–10

All the results presented are the mean values ± SD for three independent replicates
N.D., not detected; Y_X/S, growth yield coefficient, g DCW/g VFAs, Y_L/S, lipid yield coefficient, g lipid/g VFAs
^aLag phase less than 3 h was recorded as 0 day

Back to article page

ISSN: 2731-3654

Contact us

General enquiries: journalsubmissions@springernature.com