Fig. 2

LPMO activity in the presence of CDH and glucose oxidase. a Solid lines indicate the time-dependent absorbance changes of a 0.8 mg mL⁻¹ PASC solution in presence of 3 µM LPMO and 0.5, 1.0 or 3.0 µM NcCDHIIA. Reactions were carried out in 50 mM potassium phosphate buffer, pH 6.0. The same reactions were carried out in presence of 2000 U mL⁻¹ catalase (colour-coded dashed lines). Traces were vertically shifted for better visibility. b Reaction rates of PASC degradation of the reactions shown in a. c LPMO activity in the presence of 0.5 µM NcCDHIIA and various indicated activities of glucose oxidase (GOX). The volumetric activity of GOX was determined as described in the “Methods” section. All samples contained 10 mM glucose and 10 mM lactose as a substrate for GOX and CDH, respectively, and were carried out at 30 °C in 50 mM sodium phosphate buffer, pH 6.0. In control experiments, NcCDHIIA (grey dashed line) or GOX (black line) were avoided