Peak no. | Wavenumber (cm−1) | Peak assignment | Main biomolecules |
---|---|---|---|
1 | 3010 | =C–H stretching | Lipid |
2 | 2955 | C–H asymmetric stretching of –CH3 | Lipid |
3 | 2925 | Stretching of >CH2 of acyl chains (asymmetric) | Lipid |
4 | 2850 | Stretching of CH2 of acyl chains (symmetric) | Lipid |
5 | 1745 | C=O stretching | Lipid |
6 | 1680–1640 | Amide I band (C=O stretching) | Protein |
7 | 1580–1520 | Amide II (CONH bending) | Protein |
8 | 1465 | CH2 deformation | Lipid |
9 | 1410 | Amide III band (C–N stretching) | Protein |
10 | 1380 | CH3 bending | Lipid |
11 | 1240 –1265 | P=O stretching (asymmetric) of >PO2 phosphodiesters | Polyphosphate, phospholipid |
12 | 1155 | C–O–C stretching | Lipid |
13 | 1080 | P=O stretching (symmetric) of >PO2 | Polyphosphate, phospholipid |
14 | 900–1200 | C–O and C–C stretching, C–O–H and C–O–C deformation | Carbohydrate |
15 | 875 | P–O–P stretching | Polyphosphate, phospholipid |
16 | 725 | CH2 deformation | Lipid |