Fig. 5From: In silico evolution of Aspergillus niger organic acid production suggests strategies for switching acid outputEvolution plot showing the site and frequency of mutations from 10 independent runs with evolutionary pressure towards succinic acid production. Each of the ten grey circles corresponds to the results of one replicate run. The numbers on the outside are indices and refer to reactions where mutations occurred. The corresponding reactions are given in Additional file 5: Table S15. Dots on the grey circles align with these indices and indicate where mutations occurred. The diameter of each dot is proportional to the frequency of the corresponding mutation across solutions from the run. A frequency cut-off of 0.2 was applied. Mutations with a frequency lower than the cut-off are not represented. Green dots indicate mutations that when complemented decrease target acid flux by > 95%. Purple dots indicate mutations that when complemented decrease target acid flux by < 95%. The sectors indicate areas of metabolism that the mutations targetedBack to article page