Skip to main content

Table 5 Example solution from evolution of succinic acid production

From: In silico evolution of Aspergillus niger organic acid production suggests strategies for switching acid output

IndexReactionMutation effectComplementation results
% Fitness decrease% Acid flux decrease% Growth increase
444QH2m + 0.5*O2m → Qm + H2OmUC99.810098.2
449QH2m + 2*FERIm + 2*Hm → Qm + 2*FEROm + 4*HoUC99.810098.2
213PYR + NADH + H → LLAC + NADUC85.386.219.3
84LAC + NAD  PYR + NADH + HLC85.286.219.3
77H + PYR → ACAL + CO2UC77.177.89.6
265GLCNT + ATP → D6PGC + ADP + HUC52.454.412.5
24G6P + NADP → D6PGL + NADPH + HUC49.751.812.3
22ATPm + PYRm + H2Om + CO2m → ADPm + PIm + OAm + 2*HmUC18.922.514
1242NH4OH  NH3 + H2OUC12.813.83.5
  1. The example solution is chosen as the best representative of the average solution and based on fitness. The mutation effect is given as UC or LC. UC corresponds to a mutation that imposes a flux constraint on the upper bound. LC corresponds to a mutation that imposes a flux constraint on the lower bound. Complementation results are given for each mutation, showing the effect on fitness, target acid flux, and growth when the mutation is complemented with the wild-type while retaining the other mutations