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Fig. 7 | Biotechnology for Biofuels

Fig. 7

From: Metabolic and evolutionary responses of Clostridium thermocellum to genetic interventions aimed at improving ethanol production

Fig. 7

Strains LL374 (∆hptspo0Aldhpta::PgapD-cat-hpt adapted by pH auxostat), LL375 (∆hptspo0Aldhpta::PgapD-cat-hpt adapted by chemostat), LL1011 (∆hptldhpta adapted by serial transfer), LL1043 (∆hptldhpta::PgapD-cat-hpt adapted by pH auxostat) cultivated on 120 g/L cellulose. Both strains LL1011 and LL1043 have the adhED494G mutation. The left panels show residual cellulose concentration fitted with a sigmoidal curve, the rate of cellulose solubilization and pellet nitrogen concentration as proxy for cells. The middle panel figures show major fermentation products ethanol, isobutanol as well as formate and fumarate. The right panel set shows total amino acids, valine and alanine as well as pyroglutamate. For the middle and right panel sets the arrows on the top y-axis indicate maximum cell concentration (red) and maximum of cellulose solubilization (green). Values in the brackets of the legend of the left panel figures represent the maximum values of the amount cellulose utilized, cell concentration and cellulose solubilization rate (as indicated), values shown in brackets in the legends of the middle and right panels show the maximum average values measured of fermentation metabolites. All data other than the residual cellulose concentration fit curve and the solubilization rate curve are averages from duplicate fermentation runs, and error bars represent ± 1 standard deviation

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