Fig. 4

Genetic map of NsTPS with integration site of pNsShble. a Genetic map of NsTPS is shown in approximate scale showing the TPS and TPP domains, together with integration site of pNsShble. Locations of primers used for genomic PCR and qRT-PCR are shown. Amplicons of TPS1 and TPS2 were located in the TPS domain, while TPP1 and TPP2 were in the TPP domain. b Genomic DNA PCR was performed to confirm the integration site revealed by whole genome sequencing. M, marker; lane 1, pNsShble control; lane 2, WT; lane 3, Mut68