Establishing an enzyme cascade for one-pot production of α-olefins from low-cost triglycerides and oils without exogenous H 2 O 2 addition

Table 1 OleT_JE catalytic activities in two different H₂O₂-supplying systems

Substrate	H₂O₂ supply	Conversion/%^a	TTN^a
500 μM lauric acid	500 μM H₂O₂	46.5	235 ± 4
500 μM lauric acid	AldO + 0.01% glycerol	93.6	467 ± 22**
1000 μM lauric acid	1000 μM H₂O₂	18.1	181 ± 2
	AldO + 0.01% glycerol	27.3	273 ± 4*
	AldO + 0.05% glycerol	35.4	354 ± 10*
	AldO + 0.1% glycerol	38.6	386 ± 31^ns
	AldO + 0.5% glycerol	49.3	493 ± 24*
	AldO + 1% glycerol	50.1	501 ± 39*
	AldO + 5% glycerol	64.9	649 ± 23*
	AldO + 10% glycerol	69.5	695 ± 40^ns

^aReaction conditions: 1 μM OleT_JE supported by H₂O₂ or the 5 μM AldO + glycerol system, at 30 °C for 6 h. Total turnover number (TTN): mean ± standard deviation; standard deviations were derived from at least two independent experiments. Statistical analysis of the adjacent TTN data was performed using a Student’s t test (one-tailed; *P < 0.05, **P < 0.01, ns: P > 0.05, no significant; two-sample unequal variance)

ISSN: 1754-6834