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Table 1 OleTJE catalytic activities in two different H2O2-supplying systems

From: Establishing an enzyme cascade for one-pot production of α-olefins from low-cost triglycerides and oils without exogenous H2O2 addition

Substrate

H2O2 supply

Conversion/%a

TTNa

500 μM lauric acid

500 μM H2O2

46.5

235 ± 4

AldO + 0.01% glycerol

93.6

467 ± 22**

1000 μM lauric acid

1000 μM H2O2

18.1

181 ± 2

AldO + 0.01% glycerol

27.3

273 ± 4*

AldO + 0.05% glycerol

35.4

354 ± 10*

AldO + 0.1% glycerol

38.6

386 ± 31ns

AldO + 0.5% glycerol

49.3

493 ± 24*

AldO + 1% glycerol

50.1

501 ± 39*

AldO + 5% glycerol

64.9

649 ± 23*

AldO + 10% glycerol

69.5

695 ± 40ns

  1. aReaction conditions: 1 μM OleTJE supported by H2O2 or the 5 μM AldO + glycerol system, at 30 °C for 6 h. Total turnover number (TTN): mean ± standard deviation; standard deviations were derived from at least two independent experiments. Statistical analysis of the adjacent TTN data was performed using a Student’s t test (one-tailed; *P < 0.05, **P < 0.01, ns: P > 0.05, no significant; two-sample unequal variance)