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Table 1 OleTJE catalytic activities in two different H2O2-supplying systems

From: Establishing an enzyme cascade for one-pot production of α-olefins from low-cost triglycerides and oils without exogenous H2O2 addition

SubstrateH2O2 supplyConversion/%aTTNa
500 μM lauric acid500 μM H2O246.5235 ± 4
AldO + 0.01% glycerol93.6467 ± 22**
1000 μM lauric acid1000 μM H2O218.1181 ± 2
AldO + 0.01% glycerol27.3273 ± 4*
AldO + 0.05% glycerol35.4354 ± 10*
AldO + 0.1% glycerol38.6386 ± 31ns
AldO + 0.5% glycerol49.3493 ± 24*
AldO + 1% glycerol50.1501 ± 39*
AldO + 5% glycerol64.9649 ± 23*
AldO + 10% glycerol69.5695 ± 40ns
  1. aReaction conditions: 1 μM OleTJE supported by H2O2 or the 5 μM AldO + glycerol system, at 30 °C for 6 h. Total turnover number (TTN): mean ± standard deviation; standard deviations were derived from at least two independent experiments. Statistical analysis of the adjacent TTN data was performed using a Student’s t test (one-tailed; *P < 0.05, **P < 0.01, ns: P > 0.05, no significant; two-sample unequal variance)