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Table 2 α-Olefin production from 1500 μM coconut oil by CRL/OleTJE/AldO at 30 °C for 6 h under different reaction conditions

From: Establishing an enzyme cascade for one-pot production of α-olefins from low-cost triglycerides and oils without exogenous H2O2 addition

Enzyme concentrationsGlycerol content/%Total α-olefins/μMYield/g/La
3 μM OleTJE, 15 μM AldO1.5880.5 ± 1.70.20
61377.9 ± 12.40.31
101535.6 ± 82.20.34
202138.3 ± 121.90.48
6 μM OleTJE, 30 μM AldO31339.8 ± 137.40.30
61416.6 ± 281.10.32
101820.8 ± 239.50.41
202369.9 ± 65.90.53
 202206.9 ± 27.90.49b
  1. a200-μL reaction system
  2. b10-mL reaction system. Error bars represent standard deviations derived from at least two independent experiments