Establishing an enzyme cascade for one-pot production of α-olefins from low-cost triglycerides and oils without exogenous H2O2 addition

Table 2 α-Olefin production from 1500 μM coconut oil by CRL/OleT_JE/AldO at 30 °C for 6 h under different reaction conditions

Enzyme concentrations	Glycerol content/%	Total α-olefins/μM	Yield/g/L^a
3 μM OleT_JE, 15 μM AldO	1.5	880.5 ± 1.7	0.20
	6	1377.9 ± 12.4	0.31
	10	1535.6 ± 82.2	0.34
	20	2138.3 ± 121.9	0.48
6 μM OleT_JE, 30 μM AldO	3	1339.8 ± 137.4	0.30
	6	1416.6 ± 281.1	0.32
	10	1820.8 ± 239.5	0.41
	20	2369.9 ± 65.9	0.53
	20	2206.9 ± 27.9	0.49^b

^a200-μL reaction system
^b10-mL reaction system. Error bars represent standard deviations derived from at least two independent experiments

ISSN: 2731-3654